Researchers have invented a new form of secret
messaging using bacteria that make glowing proteins only under certain
conditions. In addition to being useful to spies, the new technique could also
allow companies to encode secret identifiers into crops, seeds, or other living
commodities.
The new glowing bacteria actually did grow out of
a bit of cloak-and-dagger thinking. Several years ago, the Defense Advanced
Research Projects Agency asked researchers to submit ideas for ways to encode
secret messages without the need for electronics. At the time David Walt, a
chemist at Tufts University in Medford, Massachusetts, teamed up with his
former adviser George Whitesides, a chemist at Harvard University. Together,
they came up with a way to add a variety of metal salts to a fuse that, when
lit, would give off a sequence of pulses of infrared light that encoded a
message. That got them thinking about other ways to accomplish the same thing.
And so last year they decided to try something else, using bacteria to encode
their secrets.
The new scheme replaces the fuse with seven
colonies of Escherichia coli bacteria, each given a gene for a different
fluorescent protein. When, and only when, these genes are turned on do the
bacteria make these proteins and light up. The colors, including yellow, green,
and red, vary based on which gene is expressed. All are clearly visibly
different to the naked eye. With their colorful bacterial colonies in hand, the
researchers then created a code using pairs of different colored bacteria.
Having seven colors gave them 49 combinations, which they used to encode the 26
different letters and 23 alphanumeric symbols such as "@" and
"$." They wrote a message by simply blotting pairs of colored
bacteria in rows. To "print" the message, the researchers transferred
the bacteria onto a plate containing agar, a bacterial growth medium, into
which they pressed a sheet of nitrocellulose "paper" that immobilizes
the bacteria.
At this point, the bacteria on the nitrocellulose
paper remain invisible. But the message receiver can turn on the key genes and
make the colors light up by pressing the nitrocellulose paper into an agar
plate containing a chemical trigger that activates expression of the
fluorescent proteins. (The proteins chosen to light up are ones the bacteria
don't normally use, so unless the researchers activate them, they stay
quiescent.) As long as the receiver knows which colors correspond to which
characters, the message is revealed. But Walt and his colleagues added one more
safeguard as well. Into some bacteria they inserted genes for resistance to
particular antibiotics; the idea is that only the antibiotic-resistant bacteria
are carrying the real message. If the message fell into the wrong hands, the
receiver would see a mix of colors once the genes were activated and be unable
to read it. But if the decoder added the right antibiotic, nonresistant
bacteria and their colors die away, and the message becomes clear. The first example,
reported in today's issue of the Proceedings of the National Academy of
Sciences reads "this is a bioencoded message from the walt lab @ tufts
university 2010."
"It's a really cool idea," says Kenneth
Suslick, a chemist at the University of Illinois, Urbana-Champaign. So cool in
fact that much the same idea was suggested in Spiral, a science-fiction
thriller published earlier this year by Cornell University physicist Paul
McEuen. In the book, Liam Connor, an aging fungal biologist with enemies, encodes
clues to solving a decades-old mystery by inserting genes for fluorescent
proteins in the DNA of various fungi. Walt says he hadn't heard of the book,
though as a science-fiction fan he's eager to read it. Now with the help of his
glowing bacteria, he can write a few mysteries of his own.
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